Poster Presentation 24th Annual Lorne Proteomics Symposium 2019

Characterisation of human adipose stem cells (#107)

Naomi Koh Belic 1 , Fiona Bicknell 1 , Jerran Santos 1 , Bruce Milthorpe 1 , Matt Padula 1
  1. University of Technology Sydney, Broadway, NSW, Australia

Human adipose stem cells are widely used as treatments for a plethora of disorders, despite the lack of scientific evidence in regard to their safety let alone efficacy. These are unproven stem cell treatments being offered by predatory clinics, and there are more than 60 of these clinics practicing in Australia. Our understanding of human adipose stem cells is limited, in particular very little is known about their phenotype, as their proteome has not previously been characterised. This project isolated and characterised human adipose stem cells from 10 healthy patient’s abdominal lipoaspirates. These cells were passaged to achieve a homogenous cell culture and their proteome was characterised through a comprehensive analysis of cellular proteins, extracellular vesicles and secreted cytokines. The samples were run on our Q Exactive™ Plus Orbitrap Mass Spectrometer resulting in a quantitative proteomic profile of human adipose stem cells. Identified cellular proteins provide vital insight into cellular function, while analysis of membrane bound proteins provided an extensive catalogue of cell surface markers that are useful for antibody-based assay development. The stem cell derived extracellular vesicle proteome was also examined because stem cells secrete extracellular vesicles in substantial quantities and they are known to play a significant role in cancer, injury healing and immune suppression. Secreted cellular proteins such as cytokines also facilitate cellular communication of immune signals and warrant investigation. 27 cytokines were investigated through the utilisation of a Multiplex Immunoassay. This study produced a comprehensive data set of human adipose stem cell proteins, which is a unique resource that ultimately investigates the biological phenotype of human adipose stem cells. This is an invaluable tool for researchers and clinicians as it will assist in developing this much needed understanding of human adipose stem cells.