Cannabis is an herbaceous flowering plant of the Cannabis genus (Rosale) that has been used for thousands of years to treat a wide range of medical conditions. Despite the illicit status of cannabis, the social push for the therapeutic use of cannabis extracts has steadily increased over recent years resulting in legislation legalising the cultivation of cannabis for medicinal use. This has opened the field of medicinal cannabis research. The biochemistry of cannabis is complex including phytocannabinoids, terpenes and phenolics. Each of these classes have biologically active compounds that contribute to the medical efficacy of cannabis. The most abundant of these classes are the phytocannabinoids derived from the glandular trichomes of the flowering heads of the female plant. We have undertaken the targeted and untargeted chemotypic analysis of 70 diverse cannabis strains using liquid chromatography mass spectrometry (LCMS) to characterise the major and minor cannabinoids. Multivariate hierarchical clustering has been employed to assess similarities and differences between the strains. This provides an opportunity to target strains with specific chemotypic profiles to be used in the treatment of specific medical conditions.