Poster Presentation 24th Annual Lorne Proteomics Symposium 2019

Loss of protein N-glycosylation influences peptidoglycan structure in Campylobacter jejuni  (#84)

Zeynep Sumer-Bayraktar 1 , Joel A. Cain 1 , William Klare 1 , Paula Niewold 1 , Lok Man 1 , Stuart J Cordwell 1 2
  1. School of Life and Environmental Sciences, Charles Perkins Centre, The University of Sydney, Sydney, NSW, Australia
  2. Sydney Mass Spectrometry, The University of Sydney, Sydney, NSW, Australia

Campylobacter jejuni is a Gram-negative microaerophilic bacterium that is the leading cause of food-borne gastroenteritis in developed countries. The common route of human infection is via consumption of poorly prepared or under-cooked poultry, in which C. jejuni is an asymptomatic commensal. Human disease is characterised by mild to severe inflammatory diarrhoea, vomiting and inflammation. The molecular basis for C. jejuni infection includes initial adherence to, followed by invasion of, human intestinal epithelium. Bacterial peptidoglycan (PG) is an integral component of the cell wall / membrane that is involved in pathogen colonization, host-pathogen interactions, cell structure and morphology (1), and is a major target for existing and novel antibiotics (2). C. jejuni contains a protein N-glycosylation gene (pgl) cluster that modifies membrane-associated proteins and deletion of genes in this cluster results in reduced pathogenesis (3, 4). Proteome analysis revealed several glycoproteins in the C. jejuni PG biosynthesis pathway were impacted by oligosaccharyltransferase (pglB) gene deletion. We therefore investigated whether glycosylation influenced the peptidoglycome. Wild type and pglB mutant strain PG structures were isolated and analysed using LC-ESI tandem mass spectrometry. Abundance changes and structural features of peptidoglycans in each strain were determined. Our results showed that changes in protein glycosylation impacts the structural composition of the PG layer commensurate with changes to PG-associated proteins observed at the proteome level. We also observed altered cell morphology and reduced C. jejuni pathogenesis. Unravelling PG structures and the factors involved in their biosynthesis may help in defining why N-glycosylation is critically required for C. jejuni pathogenesis.  

  1. Frirdich E et al (2014) J Biol Chem. 289(12):8007-18
  2. Katayama N et al (2003) Plant Cell Physiol. 44(7):776-81
  3. Szymanski CM et al (2002) Infect Immun. 70:2242-4
  4. Karlyshev AV et al (2004) Microbiol. 150:1957-64