Friday, 8th February 24th Annual Lorne Proteomics Symposium 2019

Thermo Fisher Scientific Annual Users Meeting

See how our mass spectrometry solutions for HRAM Orbitrap™ systems coupled with powerful software can help you be selective and rise above the noise. From leading edge biological research to translational medicine, hear from renowned guest speakers.
For our annual Lorne user meeting workshop, the aim is to present technology, with workflows that improve throughput to simplify and answer complex biological questions. Our guest speakers are three eminent researchers in their field within ANZ.
Dr Ralph Schittenhelm from Monash University will be presenting a DIA phosphoproteomic study in response to inflammation and Dr Nicholas Scott from The University of Melbourne will present on protein-protein interactions across mouse tissues.
Closing the session will be an update on the newly released FAIMS Pro in routine plasma analysis.

Topics/ key words:
Proteomics, DIA, DDA, FAIMS, Ion Mobility, clinical trial, HF-X, plasma, Fusion Lumos, Host Pathogens Interactions , Immunology, Emerging Infections

Speakers:

• Ralf Schittenhelm, Ph.D. (Director of Monash Biomedical Proteomics Facility, Melbourne)
  “A DIA-based, Phosphoproteomic Study to Decipher Pathways Involved in MCP-1/ CCR2 Chemokine Signalling” 

• Nichollas Scott, Ph.D. (Lab Head, The Peter Doherty Institute, Melbourne)
  “An atlas of protein-protein interactions across mouse tissues”

• Lung cancer proteome biomarkers and in vitro diagnostics-multivariate index assay   - Je-Yoel Cho
• Spliced HLA peptides, a novel source for immunotherapy - Pouya Faridi
• 1,566 prostate cancer proteomes generated by PCT-SWATH mass spectrometry reveal insights into patient survival and time to recurrence - Rebecca C Poulos
• Proteomic profiling of stage I – IV colorectal carcinoma specimen for early onset biomarker detection - Christoph Krisp

• Soybean proteomics: Application to elucidation of flooding- tolerant mechanisms - Setsuko Komatsu
• Proteomics for vaccine discovery: development of two potential whole-protein vaccines with efficacy against Pseudomonas aeruginosa - William Klare
• Identification and quantitation of wheat and barley α-amylase trypsin inhibitors, the triggers of non-coeliac gluten sensitivity - Utpal Bose
• SWATH-MS reveals functional differences between glycogenin 1 and 2 in yeast metabolism - Xinle Tan
• RNA binding proteins mediated post transcriptional regulation in protist stage transitions - Balu Balan

• A search for predictive biomarkers of ovine pre-partum vaginal prolapse - Stuart Brown 
• Multi-omic profiling of metabolic dysfunction caused by myocardial ischemia / reperfusion (I/R) injury - Alexander W. Rookyard
• Proteomic heterogeneity of high-grade serous ovarian carinomas - Rohan Shah
• High-throughput sample preparation for proteomic analysis using 3d-printing - Dylan J Harney
• Deep neuroproteomics reveal defective synaptic signalling in excitotoxicity - Syeda Sadia Ameen
• The sweet connections in neurotransmission – investigating sialylated glycosylation in the active zone of nerve terminals - Inga Boll

• A search for predictive biomarkers of ovine pre-partum vaginal prolapse - Stuart Brown
• Multi-omic profiling of metabolic dysfunction caused by myocardial ischemia / reperfusion (I/R) injury - Alexander W. Rookyard
• Proteomic heterogeneity of high-grade serous ovarian carinomas - Rohan Shah
• High-throughput sample preparation for proteomic analysis using 3d-printing - Dylan J Harney
• Deep neuroproteomics reveal defective synaptic signalling in excitotoxicity - Syeda Sadia Ameen
• The sweet connections in neurotransmission – investigating sialylated glycosylation in the active zone of nerve terminals - Inga Boll
• Rapid qualitative and absolute quantification of plasma based proteins using a novel scanning quadrupole DIA acquisition method - Lee A Gethings
• Glycopeptide fragmentation optimization and quantitation by multi collision energy ramp scanning quadrupole data independent acquisition - Lee A Gethings
• Multi-omic characterisation of bladder and lung carcinomas using a novel scanning quadrupole DIA acquisition method - Heather Patsiouras
• Proteomic profiling analysis of Australian seagrass, Zostera muelleri under copper stress - Nasim Shah Mohammadi
• Larval development of the barber’s pole worm is under tight post-transcriptional control - Guangxu Ma
• Chemical and random additive noise elimination (crane) - Akila Seneviratne
• Multi-omic approaches for elucidating the assembly and function of mitochondrial respiratory chain complexes - Boris Reljic
• Sexual dimorphism and epileptogenesis in developing and adult rat brains - Merridee A Wouters
• Fully automated all-in-one sample preparation for high-throughput spectral library generation and SWATH-based quantitation - Pawel Sadowski
• Tissue-specific immunopeptidome of HLA-B*57:01 in drug hypersensitivity reactions - Johanna E E Tuomisto
• Protein O-fucosyltransferase 2 -mediated O-glycosylation of MIC2 is dispensable for Toxoplasma gondii tachyzoite infection - Nichollas E Scott
• A slimy situation: using integrated label-free quantitative proteomics to uncover potential neuropeptides within the defensive slime secretion of the striped pyjama squid (Sepioloidea lineolata) - Nikeisha J Caruana
• Optimizing red blood cell protein extraction for biomarker discovery - Stephan Klatt
• How bad is my child's burn. Differences in blister fluid biochemistry to assess paediatric burn wounds - Tony J Parker
• Loss of protein N-glycosylation influences peptidoglycan structure in Campylobacter jejuni  - Zeynep Sumer-Bayraktar
• Insulin-related deaths: analysis of insulin and synthetic analogues in coronial exhibits by QE Plus Orbitrap - Nicola Beckett
• Identification of isoaspartylated peptides by electron-transfer/higher-energy collision dissociation - Clifford Young
• Mass spectrometry-based large-cohort proteomics for precision medicine – An international Cancer Moonshot multiple site study - Andreas Huhmer
• SWATH-MS proteomics enabled studying the semen quality in Brahman bulls - Rajesh Gupta
• Delineation of the monocyte chemoattractant protein-dependent CCR2 signalling network using phosphoproteomics - Simon Foster
• Profiling of ascites proteome in ovarian cancer patients - Dilys TH Leung
• A multidisciplinary approach to the analysis of complex MSI data - Matthew O'Rourke
• Improved lipid profiling workflow using novel iterative MS/MS mode and in silico annotation  - Christine Miller
• Developing an automated plasma sample preparation method for LC/MS analysis of metabolites - Christine Miller
• Chemotyping of medicinal cannabis phytocannabinoids - Aaron Elkins
• Multi-omics analysis of a nutrient transport protein required for full virulence in Campylobacter jejuni   - Lok Man
• Early detection of honey bee malnutrition and colony collapse - Clara Castaños Sánchez de la Barquera
• Improved metabolome coverage and increased confidence in unknown identification through novel automated acquisition strategy combining sequential injections and MSⁿ - Amanda Souza
• Flavonoid annotation using a product ion-dependent MSⁿ data acquisition method on a Tribrid Orbitrap mass spectrometer - Amanda Souza
• Bespoke deuterated lipid molecules for structure function applications from the National Deuteration Facility  - Karyn L Wilde
• A high throughput single platform for quantitative multiOmics - Robert Plumb
• High throughput lipidomics using ion mobility enabled rapid LC-MS profiling shows promise for the analysis of human plasma samples obtained from breast cancer patients - Robert Plumb
• An "OMICS" approach to food profiling, authenticity, and classification   - Katherine Hyland

Food analysis has been expanded to incorporate all manner of post-genomic profiling, classification, nontargeted screening, and authentication of any of a variety of food, environmental, or agricultural products.
We will demonstrate workflows utilizing SCIEX high resolution mass spectrometric technology, novel data acquisition techniques, spectral libraries, and software tools to utilize nontargeted and statistical approaches to classify such samples as juices, herbs, and cannabis strains, and identify unknown constituents in complex matrices like citrus fruits extracts and even environmental water samples. 

Speaker:

• Katherine Hyland
  Global Technical Marketing - Food and Environmental - SCIEX

• Methylation throughout the proteome: The methyltransferases tell the story - Joshua J Hamey
• Interactions between small molecules recapitulate the exercise phosphoproteome and regulate protein secretion in vitro - Elise J Needham
• Distribution of isomerized and racemized amyloid β isoforms in the Human Brain using ion-mobility mass spectrometry - Soumya Mukherjee
• The reduced protein methylation network in the early-branching protozoan parasite, Giardia duodenalis - Samantha J Emery-Corbin
• Phosphoproteomic analysis incorporating titanium dioxide - and phosphotyrosine superbinder SH2 domain-affinity purification unveils unique signaling mechanism of the tumour suppressor protein kinase CHK in colorectal cancer cells - Heung-Chin Cheng

Network with fellow delegates during a relaxed food festival style function. With three restaurants combining there will be something for everyone, including Japanese (Unisango), Indian (Jashn) and Australian (Lorne Central) cuisine. All 3 restaurants are located on the promenade.

• Spatial proteomics with translational imaging mass spectrometry - Ron M.A. Heeren

• Combining chemical biology tools with metabolomics to identify small molecule targets of peroxide antimalarials - Darren Creek
• The cannabis metabolome - Simone Rochfort
• Rapid Evaporative Ionisation Mass Spectrometry: a new tool for rapid screening of detailed composition data in food - Alastair B Ross

• Utilising metabolic profiling and inflammation markers as diagnostic tools - Robert Trengove
• Uncovering the role of brain-derived lipid exosomes in Alzheimer's disease - Gavin Reid
• Lipidr: targeted lipidomics analysis workflow in R - Ahmed Mohamed
• The developmental lipidome and proteome of Haemonchus contortus reflect unique adaptations in the transition to parasitism - Shuai Nie
• Hooked on fat: nutrient uptake in chronic lymphocytic leukaemia   - Lauren A Thurgood
• Surfaceome of exosomes secreted from the colorectal cancer cell line SW480   - Rong Xu

Proteomics 2019 & Metabolomics Symposium posters will be displayed together.
The Proteomics 2019 posters are listed in the session above titled "Lunch & Poster Session One".

• Metabolic reprogramming in cancer - Kristin K Brown
• Multi-omics analysis of esophageal adenocarcinoma reveals impaired lipid antioxidant mechanisms during disease progression - Jeffrey Molendijk
• From phenotypic screens to mode of action: A metabolomics approach to guide the development of novel anti-trypanosomal Drugs - Anubhav Srivastava

• A meta-analysis of metabolites associated with BMI in > 30,000 individuals from the COMETs consortium - Jessica A Lasky-Su